Examine the biomedical impact of vaping through physiological, molecular, and genetic lenses. Analyze real-world case studies, use an ELISA simulation to detect inflammatory and oxidative stress biomarkers, and perform gel electrophoresis analysis to explore genetic predispositions to vaping-related diseases. Integrate molecular diagnostics with public health, educators will connect environmental exposure to disease risk, gaining strategies to teach pathophysiology, prevention, and personalized health decision-making.