Pigs characterized with physiological and anatomical similarities to humans provide attractive models for investigating human diseases. Recent technological development of genome editing and somatic cell cloning enabled us to develop variety of genetically engineered pigs that reproduce human monogenic diseases. Our recent achievements in developing disease model pigs including diabetes, Duchenne muscular dystrophy, ornithine transcarbamylase deficiency, Marfan syndrome, autosomal dominant polycystic kidney disease, and dilated cardiomyopathy will be discussed.